ABSTRACT
100 cataract patients of IGGMC attending Biochemistry OPD for routine Blood sugar, were estimated for serum electrolyte level i.e. Sodium, Potassium level and compared with normal healthy age related ( 50-70 yrs) control by t test. Plasma Glucose level (to rule out Diabetes) and serum Creatinine (to rule out renal disorder) in both cases and control were also studied. Result of our study shows Elevation in serum Na level in cataract pts mean 148.52 + /-4.13 meq/lt compared to control mean 139.26 +/-3.08 meq/lt (p value 0.001) which is significantly high. Normal Serum Na level is required to maintain proper water electrolyte balance across lens membrane that in turn is also responsible for maintaining lens membrane permeability. Elevation in serum Na level in cataract pts may result into its further increase in aqueous humor of lens which may lead to osmotic imbalance across lens membrane and aggravate, progression of disease. We conclude that salt restricted Diet must be advised in Cataract patients so as to maintain normal electrolyte balance which may prevent further progression of disease.
Subject(s)
Aged , Blood Glucose/analysis , Blood Glucose/blood , Cataract/epidemiology , Cataract/metabolism , Creatinine/analysis , Creatinine/blood , Electrolytes/analysis , Electrolytes/blood , Humans , Middle Aged , Water-Electrolyte Imbalance/analysis , Water-Electrolyte Imbalance/blood , Water-Electrolyte Imbalance/diagnosisABSTRACT
Oubain sensitive and insensitive adenosine triphosphatase showed decrease in their activities in the polymorphonuclear leukocytes of obese patients while the activity of acetylcholinesterase was found to be increased significantly. The contents of sodium, potassium and magnesium were found to be significantly decreased in polymorphonuclear leukocytes of obese patients. Polymorphonuclear leukocytes obtained from treated obese patients showed considerable restoration.
Subject(s)
Acetylcholinesterase/metabolism , Adult , Appetite Depressants/therapeutic use , Body Mass Index , Ca(2+) Mg(2+)-ATPase/metabolism , Cell Membrane/enzymology , Fenfluramine/therapeutic use , Humans , Magnesium/metabolism , Male , Neutrophils/enzymology , Obesity, Morbid/drug therapy , Potassium/metabolism , Sodium/metabolism , Sodium-Potassium-Exchanging ATPase/metabolism , Spectrophotometry, AtomicABSTRACT
Male and female albino rats of same age and body weight were pair fed with laboratory stock diet and ascorbic acid, dehydroascorbic acid and diketogulonic acid were determined in the liver and urine, while in blood only ascorbic acid was estimated. Male rats had concentration higher of ascorbic acid in liver and urine as compared with females, while there were no significant variations in the contents of dehydroascorbic acid and diketogulonic acid. Hepatic and renal 2, 3-diketoaldonate decarboxylase, and hepatic dehydroascorbatase were also found to be significantly higher in male rats. Similar sex variations were also observed in ascorbic acid catabolism in guinea pigs without any differences in urinary ascorbic acid contents.
Subject(s)
Animals , Ascorbic Acid/metabolism , Catalysis , Female , Guinea Pigs , Liver/metabolism , Male , Rats , Sex CharacteristicsABSTRACT
Polymorphonuclear leukocytic (PNML) lipid content was studied in chronic alcoholic and normal control subjects. Chronic alcoholic subjects showed abnormal liver function and abnormal serum lipid profile. PMNL obtained from chronic alcoholic subjects exhibited decreased free cholesterol and phospholipid contents, with a significant increase in cholesterol to phospholipid ratio. Although, there was no change in the free fatty acids, the levels of triglycerides were significantly elevated.